| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA polymerase delta subunit 3; DNA polymerase delta subunit C; DNA polymerase delta subunit p66; DNA polymerase delta subunit p68; POLD3; KIAA0039 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human POLD3 recombinant protein (Position: D11-K296). Human POLD3 shares 87.8% amino acid (aa) sequence identity with mouse POLD3. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects DNA polymerase delta subunit 3 (gene POLD3) in human, mouse and rat samples and is validated for Western blot and ELISA.
POLD3 is an accessory subunit of DNA polymerase δ and is also part of DNA polymerase ζ. It stabilizes the polymerase δ complex and helps it respond to PCNA, the sliding clamp that keeps polymerases on DNA. POLD3 is studied in DNA replication, break-induced replication and translesion synthesis.
| Target | DNA polymerase delta subunit 3 (gene POLD3; UniProt Q15054, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human POLD3 fragment (Asp11–Lys296), expressed in E. coli. Sequence identity with the mouse ortholog: 87.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DNA polymerase delta subunit 3 in the cytoplasm and nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
