| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1; POMGnT1; UDP-GlcNAc:alpha-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I.2; GnT I.2; MGAT1.2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human POMGNT1 recombinant protein (Position: R96-E394). Human POMGNT1 shares 99.7% amino acid (aa) sequence identity with both mouse and rat POMGNT1. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (gene POMGNT1) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
POMGNT1 is a Golgi glycosyltransferase that adds N-acetylglucosamine to O-linked mannose on proteins. This builds part of the O-mannose glycans on α-dystroglycan that are needed for binding to laminin and other matrix proteins. POMGNT1 mutations cause muscle-eye-brain disease and other dystroglycanopathies.
| Target | Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (gene POMGNT1; UniProt Q8WZA1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human POMGNT1 fragment (Arg96–Glu394), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99.7%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 in the Golgi apparatus membrane. Tissue expression noted by UniProt (human): Constitutively expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
