| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2; POMGnT2; Extracellular O-linked N-acetylglucosamine transferase-like; Glycosyltransferase-like domain-containing protein 2; AGO61; C3orf39; EOGTL; GTDC2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human POMGNT2 recombinant protein (Position: R40-K378). Human POMGNT2 shares 94.7% and 95% amino acid (aa) sequence identity with mouse and rat POMGNT2, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (gene POMGNT2) in human, mouse and rat samples and is validated for Western blot and ELISA.
POMGNT2 is an ER glycosyltransferase that attaches N-acetylglucosamine in a β1,4 linkage to O-linked mannose. This is an early step in building the phosphorylated core M3 glycan on α-dystroglycan, which allows high-affinity binding to laminin. POMGNT2 mutations cause a form of congenital muscular dystrophy.
| Target | Protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 (gene POMGNT2; UniProt Q8NAT1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human POMGNT2 fragment (Arg40–Lys378), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 94.7% and 95%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Highly expressed in the brain, muscle, heart, and kidney in both fetus and adult.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
