| Field | Specification |
|---|---|
| Target | |
| Alternative names | POMT1; Protein O-mannosyl-transferase 1; EC 2.4.1.109; Dolichyl-phosphate-mannose--protein mannosyltransferase 1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human POMT1 recombinant protein (Position: H224-H747). Human POMT1 shares 81.1% and 80.9% amino acid (aa) sequence identity with mouse and rat POMT1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects protein O-mannosyl-transferase 1 (gene POMT1) in human and rat samples and is validated for Western blot, flow cytometry and ELISA.
Protein O-mannosyltransferase 1 (POMT1) works with POMT2 in the endoplasmic reticulum to attach mannose to serine or threonine residues of target proteins. Its main substrate is α-dystroglycan, and this O-mannosylation is required for dystroglycan to bind extracellular matrix. POMT1 mutations cause Walker-Warburg syndrome and related muscular dystrophies.
| Target | Protein O-mannosyl-transferase 1 (gene POMT1; UniProt Q9Y6A1, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human POMT1 fragment (His224–His747), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 81.1% and 80.9%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places protein O-mannosyl-transferase 1 in the endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
