| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serum paraoxonase/arylesterase 1; PON 1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse PON1 recombinant protein (Position: A30-D274). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 9D3 is a mouse monoclonal antibody that recognizes serum paraoxonase/arylesterase 1 (gene Pon1) in mouse and rat samples and is validated for ELISA, IHC and Western blot. The predicted molecular weight is 39.6 kDa, and the supplier reports an observed band at about 40 kDa.
Paraoxonase 1 (PON1) is a calcium-dependent esterase made in the liver and carried in blood on HDL particles. It hydrolyzes lactones and toxic organophosphate metabolites such as paraoxon, and it helps protect LDL from oxidation. PON1 is widely studied in lipid metabolism, oxidative stress, cardiovascular research and pesticide sensitivity.
| Target | Serum paraoxonase/arylesterase 1 (gene Pon1; UniProt P52430, mouse) |
|---|---|
| Clone | 9D3 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 39.6 kDa |
| Observed MW | 40 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
| ELISA (capture antibody) | 1–5 µg/mL |
Samples with a confirmed band (WB): mouse liver tissue, rat liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Recombinant mouse PON1 fragment (Ala30–Asp274), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes serum paraoxonase/arylesterase 1 as a secreted protein. Tissue expression noted by UniProt (mouse): Plasma, liver, kidney, heart, brain, small intestine and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
