| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ras-related protein Rab-5A; RAB5A; RAB5 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human PP2A-alpha recombinant protein (Position: M1-L309). Human PP2A-alpha shares 100% amino acid (aa) sequence identity with both mouse and rat PP2A-alpha. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-PP2A-alpha/PPP2CA Antibody Picoband® (monoclonal, 3B6) is an antibody for PPP2CA detection raised in Mouse (Monoclonal, clone Clone: 3B6, Mouse IgG2a), with reported reactivity: Human,Monkey,Mouse,Rat. Commonly used in WB, IHC, IF, ICC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: PPP2CA (RAB5A, member RAS oncogene family); UniProt: P67775
- Antibody format: Mouse, Monoclonal, clone Clone: 3B6, Mouse IgG2a
- Molecular weight: 36 kDa, calculated 25035 MW
- Applications: WB, IHC, IF, ICC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-PP2A-alpha/PPP2CA Antibody Picoband® (monoclonal, 3B6) catalog # M01893-1.
Biological background
Biological context: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors ly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3.
Expression and localization notes: cellular localization: Early endosome membrane. Lipid-anchor. Endosome membrane. Cytosol. Cell membrane. Cytoplasmic side. Melanosome. Cytoplasmic vesicle. Ruffle. Membrane. Phagosome membrane., tissue context: Widely expressed in fetal and adult tissues..
Common research applications
- Western blotting (WB): Compare PPP2CA levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of PPP2CA in tissue sections, considering fixation and antigen retrieval effects.
- Immunofluorescence / ICC: Assess subcellular localization patterns and co-localization with compartment markers in cultured cells.
- Flow cytometry: Quantify PPP2CA-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide. It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Early endosome membrane. Lipid-anchor. Endosome membrane. Cytosol. Cell membrane. Cytoplasmic side. Melanosome. Cytoplasmic vesicle. Ruffle. Membrane. Phagosome membrane.
- Tissue details: Widely expressed in fetal and adult tissues.
- Research category: Cell Type Marker,Neuron Marker,Neuroscience,Neurotransmission,Organelles,Protein Trafficking,Secretory Vesicles,Signal Transduction,Subcellular Markers,Synapse Marker,Tags & Cell Markers,Vesicle Transport
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
