| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphopantothenate--cysteine ligase; Phosphopantothenoylcysteine synthetase; PPC synthetase; PPCS; COAB |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PPCS |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 29P15 is a rabbit monoclonal antibody that recognizes phosphopantothenate--cysteine ligase (gene PPCS) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 34.0 kDa, and the supplier reports an observed band at about 34 kDa.
Phosphopantothenoylcysteine synthetase (PPCS) catalyzes the second step of coenzyme A synthesis from vitamin B5, joining cysteine to 4′-phosphopantothenate. Because coenzyme A is required for fatty acid metabolism and energy production, PPCS is studied in CoA biosynthesis, and its mutations cause a form of dilated cardiomyopathy.
| Target | Phosphopantothenate--cysteine ligase (gene PPCS; UniProt Q9HAB8, human) |
|---|---|
| Clone | 29P15 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.0 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): SH-SY5Y cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PPCS.
Reactivity Notes
The supplier lists reactivity with human. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
