| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein phosphatase with EF-hands 1; PPEF-1; Protein phosphatase with EF calcium-binding domain; PPEF; Serine/threonine-protein phosphatase 7; PP7; PPEF1; PPP7C |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PPEF1 recombinant protein (Position: H137-L652). Human PPEF1 shares 72.7% and 73.2% amino acid (aa) sequence identity with mouse and rat PPEF1, respectively. |
| Purification | |
| Reconstitution | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects serine/threonine-protein phosphatase with EF-hands 1 (gene PPEF1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
PPEF1 is a serine/threonine phosphatase with EF-hand calcium-binding motifs, related to the Drosophila retinal degeneration C (rdgC) protein. It has been reported to dephosphorylate and destabilize PDCD5, dampening p53 signaling, and may take part in photoreceptor adaptation. Its functions are still being characterized.
| Target | Serine/threonine-protein phosphatase with EF-hands 1 (gene PPEF1; UniProt O14829, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PPEF1 fragment (His137–Leu652), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 72.7% and 73.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Detected in retina and retinal derived Y-79 retinoblastoma cells.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
