| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; PP2Aa; Medium tumor antigen-associated 61 kDa protein; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PPP2R1A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human PPP2R1A, which shares 95.2% amino acid (aa) sequence identity with mouse PPP2R1A, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (gene PPP2R1A) in human, monkey, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 65.3 kDa, and the supplier reports an observed band at about 65 kDa.
PPP2R1A is the α isoform of the scaffold A subunit (PR65) of protein phosphatase 2A. It holds the catalytic C subunit and a variable B subunit together, so it is needed for assembling most PP2A holoenzymes. PPP2R1A is recurrently mutated in some endometrial and ovarian cancers and is studied in signaling and tumor suppression.
| Target | Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (gene PPP2R1A; UniProt P30153, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 65.3 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse, Rat) |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human A431 cells, monkey COS-7 cells, rat kidney tissue, rat C6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): 293T cells, C6 cells, RAW264.7 cells.
Immunogen
Synthetic peptide from the middle region of human PPP2R1A. Sequence identity with the mouse ortholog: 95.2%.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform in the cytoplasm, nucleus, chromosome and lateral cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Jia-ning Gong et al. (2026) Platycodin D alleviates idiopathic pulmonary fibrosis by targeting PPP2R1A and regulating the PP2A-mediated PI3K/Akt pathway to inhibit fibroblast transdifferentiation. Phytomedicine. 10.1016/j.phymed.2026.158000
