| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform; PP2A B subunit isoform B'-delta; PP2A B subunit isoform B56-delta; PP2A B subunit isoform PR61-delta; PP2A B subunit isoform R5-delta; PPP2R5D |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PPP2R5D |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30P07 is a rabbit monoclonal antibody that recognizes serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (gene PPP2R5D) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 70.0 kDa; the supplier reports observed bands at 60, 65 kDa.
PPP2R5D (B56δ) is a B′-family regulatory subunit of protein phosphatase 2A that directs its substrate specificity and location. It is highly expressed in brain, and de novo PPP2R5D variants cause a neurodevelopmental disorder, making it a focus of PP2A studies in neurons.
| Target | Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform (gene PPP2R5D; UniProt Q14738, human) |
|---|---|
| Clone | 30P07 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 70.0 kDa |
| Observed MW | 60, 65 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PPP2R5D.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta isoform in the cytoplasm and nucleus. Tissue expression noted by UniProt (human): Isoform Delta-2 is widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
