| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform; CAM-PRP catalytic subunit; Calmodulin-dependent calcineurin A subunit beta isoform; CNA beta; PPP3CB; CALNA2; CALNB; CNA2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PPP3CB recombinant protein (Position: H36-Q524). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (gene PPP3CB) in human, monkey, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 59.0 kDa, and the supplier reports an observed band at about 59 kDa.
Calcineurin Aβ (PPP3CB) is a catalytic subunit of calcineurin, the Ca2+/calmodulin-dependent phosphatase. It dephosphorylates targets such as NFATC1 and TFEB, linking calcium signals to gene expression and lysosome biogenesis. PPP3CB is studied in immune cells, heart and muscle.
| Target | Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform (gene PPP3CB; UniProt P16298, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 59.0 kDa |
| Observed MW | 59 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Monkey, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells, monkey COS-7 cells, rat brain tissue, rat heart tissue, rat C6 cells, mouse brain tissue, mouse heart tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human rectum adenocarcinoma tissue, human tonsil tissue, human urothelial carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human PPP3CB fragment (His36–Gln524), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, monkey, mouse and rat. UniProt places serine/threonine-protein phosphatase 2B catalytic subunit beta isoform in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
