| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein phosphatase 4 regulatory subunit 1; PPP4R1; MEG1; PP4R1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at C-terminus of human PPP4R1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets serine/threonine-protein phosphatase 4 regulatory subunit 1 (gene PPP4R1) in human, mouse, rat and monkey samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 107.0 kDa, and the supplier reports an observed band at about 130 kDa.
PPP4R1 is a regulatory subunit of protein phosphatase 4. The PPP4C-PPP4R1 complex has been reported to dephosphorylate HDAC3 and TRAF2, limiting TNF-induced NF-κB activation. It is also targeted by a Merkel cell polyomavirus protein, and it is studied in phosphatase regulation of inflammatory signaling.
| Target | Serine/threonine-protein phosphatase 4 regulatory subunit 1 (gene PPP4R1; UniProt Q8TF05, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat, Monkey |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 107.0 kDa |
| Observed MW | 130 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat, Monkey) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): monkey COS-7 cells, human K562 cells, rat brain tissue, rat stomach tissue, mouse brain tissue, mouse stomach tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Synthetic peptide based on the sequence of at C-terminus of human PPP4R1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human, mouse, rat and monkey. Tissue expression noted by UniProt (human): Widely expressed with high expression in cultured mesangial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
