| Field | Specification |
|---|---|
| Target | |
| Alternative names | Serine/threonine-protein phosphatase 6 catalytic subunit; PP6C; PPP6C; PPP6 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PPP6C |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27P53 is a rabbit monoclonal antibody that recognizes serine/threonine-protein phosphatase 6 catalytic subunit (gene PPP6C) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 35.1 kDa, and the supplier reports an observed band at about 32 kDa.
PPP6C is the catalytic subunit of protein phosphatase 6 (PP6), a PP2A-related serine/threonine phosphatase. PP6 dephosphorylates TAK1 to limit IL-1 and inflammatory signaling and controls Aurora A activity during mitosis. PPP6C mutations are found in melanoma, and it is studied in signaling and cell division.
| Target | Serine/threonine-protein phosphatase 6 catalytic subunit (gene PPP6C; UniProt O00743, human) |
|---|---|
| Clone | 27P53 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.1 kDa |
| Observed MW | 32 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PPP6C.
Reactivity Notes
The supplier lists reactivity with human. UniProt places serine/threonine-protein phosphatase 6 catalytic subunit in the mitochondrion and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed in all tissues tested with highest expression levels in testis, heart, kidney, brain, stomach, liver and skeletal muscle and lowest in placenta, lung colon and spleen. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
