| Field | Specification |
|---|---|
| Target | |
| Alternative names | Myeloblastin; AGP7; C-ANCA antigen; Leukocyte proteinase 3; PR-3; PR3; Neutrophil proteinase 4; NP-4; P29; Wegener autoantigen; PRTN3; MBN |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PR3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 24P69 is a rabbit monoclonal antibody that recognizes myeloblastin (gene PRTN3) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 27.8 kDa, and the supplier reports an observed band at about 28 kDa.
Proteinase 3 (PRTN3, myeloblastin) is a neutrophil serine protease stored in azurophil granules alongside neutrophil elastase. It degrades extracellular matrix proteins and processes signaling molecules, and it is the main target of anti-neutrophil cytoplasmic antibodies (c-ANCA) in granulomatosis with polyangiitis, making it important in vasculitis research.
| Target | Myeloblastin (gene PRTN3; UniProt P24158, human) |
|---|---|
| Clone | 24P69 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 27.8 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human PR3.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes myeloblastin as secreted and also located in the cytoplasmic granule and cell membrane. Tissue expression noted by UniProt (human): Expressed in polymorphonuclear leukocytes (at protein level).
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
