| Field | Specification |
|---|---|
| Target | |
| Alternative names | Peroxiredoxin-6;1.11.1.15;1-Cys peroxiredoxin;1-Cys PRX;24 kDa protein;Acidic calcium-independent phospholipase A2;aiPLA2;3.1.1.-;Antioxidant protein 2;Liver 2D page spot 40;Non-selenium glutathione peroxidase;NSGPx;1.11.1.9;Red blood cells page spot 12;PRDX6;AOP2, KIAA0106; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 6I8 is a mouse monoclonal antibody that recognizes peroxiredoxin-6 (gene PRDX6) in human and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 25.0 kDa, and the supplier reports an observed band at about 29 kDa..
Peroxiredoxin 6 (PRDX6) is a bifunctional enzyme that combines glutathione-dependent peroxidase activity with phospholipase A2 activity. It can repair oxidized phospholipids in membranes and also acts as a lysophosphatidylcholine acyltransferase, which makes it important in lung surfactant metabolism, membrane repair and oxidative stress research.
| Target | Peroxiredoxin-6 (gene PRDX6; UniProt P30041, human) |
|---|---|
| Clone | 6I8 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2b |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 25.0 kDa |
| Observed MW | 29 kDa. |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human hela tissue, human Jurkat cells, human HEK293 cells, human K562 cells, human SW620 cells, rat RH35 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human endometrial carcinoma tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A549 Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins The cells.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Recombinant human Peroxiredoxin 6 fragment (Glu15–Pro224), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 90% and 91%, respectively.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places peroxiredoxin-6 in the cytoplasm and lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
