| Field | Specification |
|---|---|
| Target | |
| Alternative names | Transthyretin;Prealbumin;TBPA;Ttr;Tt; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived rat Prealbumin recombinant protein (Position: G21-N147). Rat Prealbumin shares 94.5% amino acid (aa) sequence identity with mouse Prealbumin. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects transthyretin (gene Ttr) in mouse and rat samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 15.7 kDa, and the supplier reports an observed band at about 16 kDa.
Transthyretin (TTR) is a tetrameric transport protein made mainly by the liver and the choroid plexus. It carries thyroxine in blood and cerebrospinal fluid and transports retinol by binding retinol-binding protein. Destabilized TTR can misfold into amyloid fibrils, causing transthyretin amyloidosis.
| Target | Transthyretin (gene Ttr; UniProt P02767, rat) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 15.7 kDa |
| Observed MW | 16 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Mouse, Rat) |
Samples with a confirmed band (WB): rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse pancreas tissue, rat kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): mouse pancreas tissue, rat kidney tissue.
Immunogen
Recombinant rat Prealbumin fragment (Gly21–Asn147), expressed in E. coli. Sequence identity with the mouse ortholog: 94.5%.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt describes transthyretin as a secreted protein. Tissue expression noted by UniProt (rat): Detected in serum and cerebrospinal fluid (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Chenming Zhang et al. (2022) Transcriptomics and proteomics analysis to explore the mechanism of Yishen Tongluo formula repairing sperm DNA damage in rats. Andrologia. 10.1111/and.14582
