| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; P-Rex1; PtdIns(3,4,5)-dependent Rac exchanger 1; PREX1; KIAA1415 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PREX1 recombinant protein (Position: N643-D1155). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (gene PREX1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 186.2 kDa, and the supplier reports an observed band at about 186 kDa.
P-Rex1 (PREX1) is a guanine nucleotide exchange factor that activates Rac GTPases. It is switched on jointly by PIP3 produced by PI3K and by Gβγ subunits released from activated G protein-coupled receptors, and it is studied in neutrophil migration, cytoskeletal remodeling and cancer cell invasion.
| Target | Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein (gene PREX1; UniProt Q8TCU6, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 186.2 kDa |
| Observed MW | 186 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg /1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): MCF-7 cells.
Immunogen
Recombinant human PREX1 fragment (Asn643–Asp1155), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein in the cytoplasm and cell membrane. Tissue expression noted by UniProt (human): Mainly expressed in peripheral blood leukocytes and brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
