| Field | Specification |
|---|---|
| Target | |
| Alternative names | cAMP-dependent protein kinase type I-alpha regulatory subunit; Tissue-specific extinguisher 1; TSE1; PRKAR1A; PKR1; PRKAR1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PRKAR1A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 20P51 is a rabbit monoclonal antibody that recognizes cAMP-dependent protein kinase type I-alpha regulatory subunit (gene PRKAR1A) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 43.0 kDa, and the supplier reports an observed band at about 48 kDa.
PKA RIα (PRKAR1A) is the main type I regulatory subunit of cAMP-dependent protein kinase A. It holds the catalytic subunits inactive until cAMP binds and releases them, and it is a well-known tumor suppressor gene whose loss underlies Carney complex, so it is widely studied in cAMP signaling and endocrine research.
| Target | CAMP-dependent protein kinase type I-alpha regulatory subunit (gene PRKAR1A; UniProt P10644, human) |
|---|---|
| Clone | 20P51 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 43.0 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): U-87MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PRKAR1A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cAMP-dependent protein kinase type I-alpha regulatory subunit in the cell membrane. Tissue expression noted by UniProt (human): Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
