| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interferon-inducible double-stranded RNA-dependent protein kinase activator A; PKR-associated protein X; PKR-associating protein X; Protein activator of the interferon-induced protein kinase; Protein kinase, interferon-inducible double-stranded RNA-dependent activator; PRKRA; PACT; RAX |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PRKRA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23P32 is a rabbit monoclonal antibody that recognizes interferon-inducible double-stranded RNA-dependent protein kinase activator A (gene PRKRA) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 34.4 kDa, and the supplier reports an observed band at about 34 kDa.
PACT (PRKRA) is a double-stranded RNA-binding protein that can activate the kinase PKR in response to cellular stress, leading to phosphorylation of eIF2α and a shutdown of translation. It also partners with Dicer in small RNA processing, and PRKRA mutations are associated with an inherited form of dystonia.
| Target | Interferon-inducible double-stranded RNA-dependent protein kinase activator A (gene PRKRA; UniProt O75569, human) |
|---|---|
| Clone | 23P32 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.4 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PRKRA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places interferon-inducible double-stranded RNA-dependent protein kinase activator A in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
