| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein arginine N-methyltransferase 1;2.1.1.319 ;Histone-arginine N-methyltransferase PRMT1;Interferon receptor 1-bound protein 4;PRMT1;HMT2, HRMT1L2, IR1B4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PRMT1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAGF-16 is a rabbit monoclonal antibody that recognizes protein arginine N-methyltransferase 1 (gene PRMT1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 42.5 kDa, and the supplier reports an observed band at about 42 kDa.
PRMT1 is the main type I protein arginine methyltransferase in mammalian cells, producing monomethyl and asymmetric dimethyl arginine. It deposits the activating histone mark H4R3me2a and methylates many RNA-binding and signaling proteins, influencing transcription, RNA processing and the DNA damage response, and it is studied in cancer and development.
| Target | Protein arginine N-methyltransferase 1 (gene PRMT1; UniProt Q99873, human) |
|---|---|
| Clone | AAGF-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 42.5 kDa |
| Observed MW | 42 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:1000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:100–1:500 |
Immunogen
Synthetic peptide based on the sequence of human PRMT1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein arginine N-methyltransferase 1 in the nucleus, cytoplasm and lysosome membrane. Tissue expression noted by UniProt (human): Widely expressed.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
