| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-10;CASP-10;3.4.22.63;Apoptotic protease Mch-4;FAS-associated death domain protein interleukin-1B-converting enzyme 2;FLICE2;ICE-like apoptotic protease 4;Caspase-10 subunit p23/17;Caspase-10 subunit p12;CASP10;MCH4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human pro Caspase 10 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GAF-3 is a rabbit monoclonal antibody that recognizes caspase-10 (gene CASP10) in human and mouse samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 59.0 kDa, and the supplier reports an observed band at about 70 kDa.
Caspase-10 is an initiator caspase closely related to caspase-8 that is present in humans but not in mice. It is recruited through FADD to death receptors such as Fas and TNFR1 and can cleave downstream effector caspases, contributing to extrinsic apoptosis. Mutations in CASP10 are linked to autoimmune lymphoproliferative syndrome.
| Target | Caspase-10 (gene CASP10; UniProt Q92851, human) |
|---|---|
| Clone | GAF-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.0 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:10 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (ICC/IF): MCF-7 cells.
Immunogen
Synthetic peptide based on the sequence of human pro Caspase 10.
Reactivity Notes
The supplier lists reactivity with human and mouse. Tissue expression noted by UniProt (human): Detectable in most tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
