| Field | Specification |
|---|---|
| Target | |
| Alternative names | Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human pro Caspase 3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOB-3 is a rabbit monoclonal antibody that recognizes caspase-3 (gene CASP3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 30 kDa.
Caspase-3 is the major executioner caspase of apoptosis. Activated by initiator caspases such as caspase-8 and caspase-9, it cleaves hundreds of cellular proteins, including PARP1, leading to the typical features of apoptotic cell death. Cleaved (active) caspase-3 is one of the most widely used apoptosis markers in Western blot and immunohistochemistry.
| Target | Caspase-3 (gene CASP3; UniProt P42574, human) |
|---|---|
| Clone | AOB-3 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.6 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HeLa cells, human Jurkat cells, human HepG2 cells, human 293T cells, rat C6 cells, rat RH35 cells, mouse NIH/3T3 cells, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A673 cells.
Immunogen
Synthetic peptide based on the sequence of human pro Caspase 3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places caspase-3 in the cytoplasm. Tissue expression noted by UniProt (human): Highly expressed in lung, spleen, heart, liver and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mengchao Ding et al. (2022) “Cocktail” anti-tumor strategy through enhanced antigen exposure with photothermal/chemodynamic therapy. Chemical Engineering Journal. 10.1016/j.cej.2022.141091
Wen Tang et al. (2025) Redox-sensitive hyaluronic acid-based glycopeptide nanoparticles: Dual-action platform for mitochondrial disruption and tumor suppression. International Journal of Biological Macromolecules. 10.1016/j.ijbiomac.2025.148544
Yan Wang et al. (2018) Exosomes Derived from miR-214-Enriched Bone Marrow-Derived Mesenchymal Stem Cells Regulate Oxidative Damage in Cardiac Stem Cells by Targeting CaMKII. Oxidative Medicine and Cellular Longevity. 10.1155/2018/4971261
Zhang Guomei et al. (2023) Water Extract of Rice False Smut Balls Activates Nrf2/HO-1 and Apoptosis Pathways, Causing Liver Injury. Rice Science. 10.1016/j.rsci.2023.04.003
Jing-Jing Xing et al. (2019) Supplementation of Saponins from Leaves of Panax quinquefolius Mitigates Cisplatin-Evoked Cardiotoxicity via Inhibiting Oxidative Stress-Associated Inflammation and Apoptosis in Mice. Antioxidants. 10.3390/antiox8090347
Özlem Kaplan et al. (2024) Development of recombinant protein-based nanoparticle systems for inducing tumor cell apoptosis: In vitro evaluation of their cytotoxic and apoptotic effects on cancer cells. Journal of Drug Delivery Science and Technology. 10.1016/j.jddst.2024.105565
Shiyu Gao et al. (2022) Qingxin kaiqiao fang ameliorates memory impairment and inhibits apoptosis in APP/PS1 double transgenic mice through the MAPK pathway. Drug Design Development and Therapy. 10.2147/DDDT.S188505
Wen Bao et al. (2022) Applications of daunorubicin-loaded PLGA-PLL-PEG-Tf nanoparticles in hematologic malignancies: an in vitro and in vivo evaluation. Drug Design Development and Therapy. 10.2147/DDDT.S195832
Xinjian Zhu et al. (2020) COX-2-PGE2 signaling pathway contributes to hippocampal neuronal injury and cognitive impairment in PTZ-kindled epilepsy mice. International Immunopharmacology. 10.1016/j.intimp.2020.106801
Yunpeng Zhu et al. (2021) The Delivery Materials with Chemotherapy Drugs for Treatment of the Positive Margin in Solid Tumors. Tissue Engineering Part A. 10.1089/ten.tea.2020.0076
