| Field | Specification |
|---|---|
| Target | |
| Alternative names | Progesterone receptor;PR;Nuclear receptor subfamily 3 group C member 3;PGR;NR3C3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Progesterone Receptor recombinant protein (Position: M595-K933). Human Progesterone Receptor shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Progesterone Receptor, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets progesterone receptor (gene PGR) in human samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 99.0 kDa; the supplier reports observed bands at 90 kDa (PR-A), 118 kDa (PR-B).
The progesterone receptor (PGR) is a ligand-activated nuclear receptor that controls gene expression in response to progesterone. Its two main isoforms, PR-A and PR-B, have partly different activities, and signaling through them regulates the uterus, ovary and mammary gland. PR status is routinely assessed in breast cancer research.
| Target | Progesterone receptor (gene PGR; UniProt P06401, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 99.0 kDa |
| Observed MW | 90 kDa (PR-A), 118 kDa (PR-B) |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human T-47D cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.1 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human mammary cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with confirmed staining (ICC/IF): T-47D cells.
Immunogen
Recombinant human Progesterone Receptor fragment (Met595–Lys933), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95% and 94%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places progesterone receptor in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xiaojun Chen et al. (2009) Aberrant survivin expression in endometrial hyperplasia: another mechanism of progestin resistance. Modern Pathology. 10.1038/modpathol.2009.25
Wenjuan Feng et al. (2022) Effect of Oral versus Vaginal Administration of Estradiol and Dydrogesterone on the Proliferative and Secretory Transformation of Endometrium in Patients with Premature Ovarian Failure and Preparing for Assisted Reproductive Technology. Drug Design Development and Therapy. 10.2147/DDDT.S297236
Tan Yi et al. (2005) A Model for Implantation: Coculture of Blastocysts and Uterine Endometrium in Mice. Biology of Reproduction. 10.1095/biolreprod.104.032821
Meijun SONG et al. (2023) FABP4 mediates endoplasmic reticulum stress and autophagy to regulate endometrial epithelial cell function during early sheep gestation. Journal of Reproduction and Development. 10.1262/jrd.2023-015
Kun Yin et al. (2016) Overexpression of C35 in breast carcinomas is associated with tumor progression and lymphnode metastasis. BioScience Trends. 10.5582/bst.2015.01161
Xu Wangming et al. (2011) Impact of luteinized unruptured follicles on endometrial receptivity. Journal of Huazhong University of Science and Technology-Medical Sciences. 10.1007/s11596-011-0248-z
