| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Prolactin;PRL;PRL; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human Prolactin recombinant protein (Position: L29-227C). Human Prolactin?shares?60%?and 63% amino?acid?(aa)?sequence?identity?with mouse?and?rat Prolactin, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PRL (Prolactin) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-Prolactin/PRL Antibody Picoband® catalog # PB9361. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E.coli-derived human Prolactin recombinant protein (Position: L29-227C). Human Prolactin?shares?60%?and 63% amino?acid?(aa)?sequence?identity?with mouse?and?rat Prolactin, respectively. (reported region: L29-227).
- Molecular weight context: reported MW: 25 kDa; calculated MW: 25876 MW
- Reactivity: Human
- Applications: ELISA, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Prolactin; Prolactin. Prolactin (PRL), also known as luteotropic hormone or luteotropin, is a protein that in humans is best known for its role in enabling female mammals to produce milk. This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. Functional note: Prolactin acts primarily on the mammary gland by promoting lactation. Reported localization: Secreted. Expression/tissue context: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. .
Research relevance and current trends
- Growth Factors/Hormones: Researchers commonly examine how PRL (Prolactin) relates to this theme using model systems and orthogonal readouts.
- Lipid Metabolism: Researchers commonly examine how PRL (Prolactin) relates to this theme using model systems and orthogonal readouts.
- Metabolism: Researchers commonly examine how PRL (Prolactin) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PRL (Prolactin) levels across conditions; band patterns may reflect isoforms and processing.
- ELISA-compatible use: when applicable, interpret signal as relative abundance across sample sets with consistent handling and dilution strategy.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Family / similarity context: Belongs to the somatotropin/prolactin family.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
