| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prolactin;PRL;PRL; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human Prolactin recombinant protein (Position: L29-227C). Human Prolactin shares 60% and 63% amino acid (aa) sequence identity with mouse and rat Prolactin, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes prolactin (gene PRL) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 25.9 kDa, and the supplier reports an observed band at about 25 kDa.
Prolactin (PRL) is a peptide hormone secreted mainly by lactotroph cells of the anterior pituitary. Its best-known role is stimulating mammary gland development and milk production, but it also influences reproduction, metabolism and immune function, and serum prolactin is routinely measured in endocrinology research.
| Target | Prolactin (gene PRL; UniProt P01236, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 25.9 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
E. coli-derived human Prolactin recombinant protein (Position: L29–227C). Human Prolactin shares 60% and 63% amino acid (aa) sequence identity with mouse and rat Prolactin, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes prolactin as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Naoto Tani et al. (2024) Effects of Prolactin on Brain Neurons under Hypoxia. Life-Basel. 10.3390/life14010152
Wen-wen Lin et al. (2024) Neuregulin 4 (Nrg4) cooperates with melatonin to regulate the PRL expression via ErbB4/Erk signaling pathway as a potential prolactin (PRL) regulator. Journal of Cellular Biochemistry. 10.1002/jcb.30551
Xianglong Meng et al. (2021) Metabolomics and Network Pharmacology-Based Investigation into the Mechanisms Underlying the Therapeutic Effect of a New Chinese Traditional Medicine (Cui Nai Ling) on Bromocriptine-Induced Hypogalactia. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/8857449
Fengying Huang et al. (2013) Effect of GnRH-II on the ESC proliferation, apoptosis and VEGF secretion in patients with endometriosis in vitro. International Journal of Clinical and Experimental Pathology. PMID: 24228111
Fengying Huang et al. (2013) MAPK/ERK signal pathway involved expression of COX-2 and VEGF by IL-1β induced in human endometriosis stromal cells in vitro. International Journal of Clinical and Experimental Pathology. PMID: 24133591
L Zhang et al. (2017) miR-182 selectively targets HOXA10 in goat endometrial epithelium cells in vitro. Reproduction in Domestic Animals. 10.1111/rda.13031
Yue Zhang et al. (2017) Effects of interferon tau on endometrial epithelial cells in caprine in vitro. Gene Expression Patterns. 10.1016/j.gep.2017.06.010
