| Field | Specification |
|---|---|
| Target | |
| Alternative names | Junctional adhesion molecule A;JAM-A;Junctional adhesion molecule 1;JAM-1;Platelet F11 receptor;Platelet adhesion molecule 1;PAM-1;CD321;F11R;JAM1, JCAM;UNQ264/PRO301; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PAP ( prostatic acid phosphatase) |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFFB-1 is a rabbit monoclonal antibody that recognizes prostatic acid phosphatase (gene ACP3) in human samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 44.6 kDa, and the supplier reports an observed band at about 54 kDa.
Prostatic acid phosphatase (ACP3, PAP) is highly expressed in the prostate and secreted into seminal fluid, while a membrane-bound form also exists. It acts as a phosphatase at acidic pH, and it is a classic marker of prostate tissue, also studied in pain signaling.
| Target | Prostatic acid phosphatase (gene ACP3; UniProt P15309, human) |
|---|---|
| Clone | AFFB-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.6 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): human prostate cancer.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PAP ( prostatic acid phosphatase).
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
