| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pulmonary surfactant-associated protein B; SP-B; 18 kDa pulmonary-surfactant protein; 6 kDa protein; Pulmonary surfactant-associated proteolipid SPL (Phe); SFTPB; SFTP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human SFTPB, which shares 80% amino acid (aa) sequence identity with both mouse and rat SFTPB. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets pulmonary surfactant-associated protein B (gene SFTPB) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 42.1 kDa, and the supplier reports an observed band at about 46 kDa.
Pulmonary surfactant-associated protein B (SP-B, SFTPB) is a small hydrophobic protein that helps spread and stabilize the surfactant film lining the alveoli. By lowering surface tension it keeps airspaces from collapsing at the end of breathing out, and inherited SP-B deficiency causes fatal neonatal respiratory distress.
| Target | Pulmonary surfactant-associated protein B (gene SFTPB; UniProt P07988, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 42.1 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): human MCF-7 cells, human COLO-320 cells, human HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human SFTPB. Sequence identity with the mouse and rat orthologs: 80%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhao-Hui Liu et al. (2015) 3,4-dihydroxyphenylethanol suppresses irradiation-induced pulmonary fibrosis in adult rats. International Journal of Clinical and Experimental Pathology. PMID: 26097528
Hua You et al. (2014) The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats. International Journal of Molecular Medicine. 10.3892/ijmm.2014.1745
