| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prothrombin; Coagulation factor II; F2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Prothrombin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACBD-6 is a rabbit monoclonal antibody that recognizes prothrombin (gene F2) in human samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 70.0 kDa, and the supplier reports an observed band at about 75 kDa.
Prothrombin (F2) is the vitamin K-dependent precursor of thrombin, the central protease of blood coagulation. Thrombin converts fibrinogen to fibrin, activates factors V, VIII, XI and XIII and platelets through PAR receptors, and with thrombomodulin activates the anticoagulant protein C. The prothrombin G20210A variant is a well-known risk factor for venous thrombosis.
| Target | Prothrombin (gene F2; UniProt P00734, human) |
|---|---|
| Clone | ACBD-6 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 70.0 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human serum.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human prothrombin.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes prothrombin as a secreted protein. Tissue expression noted by UniProt (human): Expressed by the liver and secreted in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
