Anti-PRRSV GP2 Polyclonal Antibody

What it means: Target names the antigen the antibody was raised against and detects. For this product the target is GP2, the minor envelope glycoprotein encoded by ORF2 of PRRSV (also written GP2a).

Why it matters:

• GP2 forms a heterotrimer with GP3 and GP4 that engages the macrophage receptor CD163 during viral entry, so it is central to entry and receptor-blocking studies.
• Selecting the right envelope glycoprotein matters: GP2, GP3, GP4 and GP5 are distinct proteins and antibodies against them are not interchangeable.
• A defined single target keeps Western blot and IHC interpretation straightforward, since only GP2-derived material should generate signal.

How to interpret here: A Target of GP2 means this antibody reports on ORF2-encoded envelope glycoprotein and will not detect GP3, GP4, GP5 or the N protein.

Tip: If you need a positive control, use the matching recombinant PRRSV GP2 protein rather than whole virus lysate, where GP2 is present at low abundance.

What it means: Clonality describes whether the antibody is a single clone or a mixed antibody population. This product is Polyclonal, meaning it contains antibodies recognising several epitopes on GP2.

Why it matters:

• A polyclonal pool binds multiple epitopes at once, which raises sensitivity and tolerates partial epitope loss from fixation or denaturation.
• Multi-epitope binding is an advantage for detecting low-abundance viral antigen but can raise background compared with a monoclonal.
• Lot-to-lot variation is inherent to polyclonal antibodies, so re-titrate when moving to a new lot.

How to interpret here: Polyclonal here means robust detection across ELISA, IHC and Western blot, at the cost of some batch variability.

Tip: Keep a reference sample or recombinant antigen aside so a new lot can be benchmarked against the old one.

What it means: Host is the species in which the antibody was raised. This antibody was raised in Rabbit, which determines the secondary antibody and the controls your assay needs.

Why it matters:

• Detection requires an anti-rabbit secondary conjugate matched to your readout (HRP, AP or fluorophore).
• In multiplex staining, the host species must differ between primaries to avoid secondary cross-detection.
• For porcine tissue work, a rabbit host avoids the anti-mouse background sometimes seen with mouse primaries on mammalian tissue.

How to interpret here: A Rabbit host means you should pair this antibody with an anti-rabbit IgG secondary and, where relevant, a normal rabbit IgG isotype control.

Tip: When co-staining with another rabbit primary, plan a sequential protocol or switch one primary to a different host.

What it means: Reactivity lists the species or pathogens the antibody is confirmed to detect. Here reactivity is PRRSV2 — porcine reproductive and respiratory syndrome virus 2, the North American genotype.

Why it matters:

• PRRSV-1 and PRRSV-2 differ substantially in envelope glycoprotein sequence, so genotype coverage cannot be assumed from one to the other.
• Confirming reactivity before a study avoids negative results caused by genotype mismatch rather than by absence of antigen.
• Field isolates vary; a strain distant from the immunogen sequence may give weaker signal.

How to interpret here: Reactivity of PRRSV2 means samples from PRRSV-1 infections, or from other arteriviruses, are outside the validated scope and need in-house confirmation.

Tip: Record the isolate or strain used in your experiments — it is the single most useful piece of information when comparing signal across laboratories.

What it means: Isotype describes the immunoglobulin class of the purified antibody. This product is IgG, the standard class for research immunoassays.

Why it matters:

• IgG binds Protein A and Protein G, so it is compatible with standard purification, immunoprecipitation and bead-capture workflows.
• Nearly all commercial anti-rabbit secondary reagents are raised against rabbit IgG, so detection reagent choice is straightforward.
• The matching isotype control for background assessment is normal rabbit IgG.

How to interpret here: An IgG isotype means this antibody drops into conventional ELISA, IHC and Western blot protocols without special handling, and any anti-rabbit IgG detection reagent already on your bench should work with it.

Tip: Use a normal rabbit IgG at the same concentration as your negative control rather than buffer alone - a buffer-only well reports plate background, not the non-specific binding that an immunoglobulin of the same class produces in your sample matrix.

What it means: Application(s) lists the assay formats the manufacturer has validated. For this antibody the validated set is ELISA, IHC, WB.

Why it matters:

• Each format stresses the epitope differently — denaturing in Western blot, fixed and retrieved in IHC, near-native in ELISA — so validation in one does not guarantee another.
• The supplied dilution ranges differ by more than an order of magnitude between formats, so always start from the format-specific recommendation.
• Formats not listed (for example flow cytometry or immunoprecipitation) have not been tested and require your own validation.

How to interpret here: ELISA, IHC, WB means you can move directly to the recommended starting dilutions for those three formats; anything else is exploratory.

Tip: Run a dilution series on a known positive sample the first time you use a new format rather than adopting the mid-point of the recommended range blindly.

What it means: Conjugate(s) states whether a label is chemically attached to the antibody. This product is Unconjugated — supplied as purified antibody with no enzyme or fluorophore attached.

Why it matters:

• An unconjugated primary gives you free choice of detection chemistry and lets signal be amplified through the secondary antibody.
• It requires a labelled anti-rabbit secondary, which must be factored into protocol time and into multiplex panel design.
• Direct-labelled formats are only needed when secondary cross-reactivity cannot be avoided.

How to interpret here: Unconjugated means detection depends on a separately purchased anti-rabbit secondary conjugate matched to your instrument.

Tip: If you need a directly labelled version for a multiplex panel, ask us about custom conjugation.