| Field | Specification |
|---|---|
| Target | |
| Alternative names | Trypsin-2; Anionic trypsinogen; Serine protease 2; Trypsin II; PRSS2; TRY2; TRYP2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PRSS2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25P92 is a rabbit monoclonal antibody that recognizes trypsin-2 (gene PRSS2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 26.5 kDa, and the supplier reports an observed band at about 30 kDa.
Trypsin-2 (PRSS2, anionic trypsinogen) is a pancreatic serine protease secreted as an inactive zymogen and activated in the small intestine to digest dietary proteins. It is also expressed in Paneth cells, where it may process defensins, and its levels are studied in pancreatitis research.
| Target | Trypsin-2 (gene PRSS2; UniProt P07478, human) |
|---|---|
| Clone | 25P92 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.5 kDa |
| Observed MW | 30 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human pancreas.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PRSS2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes trypsin-2 as a secreted protein. Tissue expression noted by UniProt (human): Expressed in Paneth cells, at the base of small intestinal crypts. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
