| Field | Specification |
|---|---|
| Target | |
| Alternative names | Prostate-specific antigen;PSA;3.4.21.77;Gamma-seminoprotein;Seminin;Kallikrein-3;P-30 antigen;Semenogelase;KLK3;APS; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PSA/KLK3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone GDC-11 is a rabbit monoclonal antibody that recognizes prostate-specific antigen (gene KLK3) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 28.7 kDa, and the supplier reports an observed band at about 34 kDa.
Prostate-specific antigen (PSA, KLK3) is a chymotrypsin-like serine protease secreted by prostate epithelial cells into seminal fluid. It cleaves the semenogelins of the seminal clot, allowing semen to liquefy and sperm to move. PSA levels in blood are widely used in prostate cancer screening and monitoring, making it one of the best-known kallikreins.
| Target | Prostate-specific antigen (gene KLK3; UniProt P07288, human) |
|---|---|
| Clone | GDC-11 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.7 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:1000–1:5000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human prostate carcinoma tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PSA/KLK3.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes prostate-specific antigen as a secreted protein. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
