| Field | Specification |
|---|---|
| Target | |
| Alternative names | Disks large homolog 4;Postsynaptic density protein 95;PSD-95;Synapse-associated protein 90;SAP-90;SAP90;DLG4;PSD95; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human PSD95, identical to the related rat and mouse sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects disks large homolog 4 (gene DLG4) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 80.5 kDa, and the supplier reports an observed band at about 90–100 kDa.
PSD-95 (encoded by DLG4) is the most abundant scaffold protein of the excitatory postsynaptic density. It binds NMDA receptors, AMPA receptor-associated proteins, potassium channels and signaling enzymes, anchoring them at the synapse. PSD-95 is widely used as a marker of excitatory synapses and studied in synaptogenesis, synaptic plasticity and neurological disease.
| Target | Disks large homolog 4 (gene DLG4; UniProt P78352, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 80.5 kDa |
| Observed MW | 90–100 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Rat) |
Samples with a confirmed band (WB): rat brain tissue, mouse brain tissue, human U-87MG cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): rat brain tissue.
Immunogen
Synthetic peptide from the C-terminal region of human PSD95. The peptide sequence is conserved in rat and mouse.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places disks large homolog 4 in the cell membrane, postsynaptic density and synapse. Tissue expression noted by UniProt (human): Brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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