| Field | Specification |
|---|---|
| Target | |
| Alternative names | Disks large homolog 4;Postsynaptic density protein 95;PSD-95;Synapse-associated protein 90;SAP-90;SAP90;DLG4;PSD95; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PSD95 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DAF-4 is a rabbit monoclonal antibody that recognizes disks large homolog 4 (gene DLG4) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 80.5 kDa, and the supplier reports an observed band at about 95 kDa.
PSD-95 (encoded by DLG4) is the most abundant scaffold protein of the excitatory postsynaptic density. It binds NMDA receptors, AMPA receptor-associated proteins, potassium channels and signaling enzymes, anchoring them at the synapse. PSD-95 is widely used as a marker of excitatory synapses and studied in synaptogenesis, synaptic plasticity and neurological disease.
| Target | Disks large homolog 4 (gene DLG4; UniProt P78352, human) |
|---|---|
| Clone | DAF-4 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 80.5 kDa |
| Observed MW | 95 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): rat brain tissue, rat C6 cells, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U87-MG cells.
Immunogen
Synthetic peptide based on the sequence of human PSD95.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places disks large homolog 4 in the cell membrane, postsynaptic density and synapse. Tissue expression noted by UniProt (human): Brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Huimao Liu et al. (2026) Resveratrol Attenuates Neuroinflammation and Gut–Brain Axis Dysfunction Induced by Lead and Cadmium Co-Exposure by Modulating Gut Microbiota in Rats. Phytotherapy Research. 10.1002/ptr.70301
Yanqiang Qi et al. (2024) Safflower Yellow Alleviates Cognitive Impairment in Mice by Modulating Cholinergic System Function, Oxidative Stress, and CREB/BDNF/TrkB Signaling Pathway. Journal of Ethnopharmacology. 10.1016/j.jep.2024.118986
Wen Yuxin et al. (2024) Esketamine Prevents Postoperative Emotional and Cognitive Dysfunction by Suppressing Microglial M1 Polarization and Regulating the BDNF-TrkB Pathway in Ageing Rats with Preoperative Sleep Disturbance. Molecular Neurobiology. 10.1007/s12035-023-03860-4
B. Chen et al. (2009) Neuroprotective effect of grafting GDNF gene-modified neural stem cells on cerebral ischemia in rats. Brain Research. 10.1016/j.brainres.2009.05.100
