| Field | Specification |
|---|---|
| Target | |
| Alternative names | P-selectin glycoprotein ligand 1; PSGL-1; Selectin P ligand; CD162; Selplg; Selp1; Selpl |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse PSGL-1/Selplg recombinant protein (Position: L18-P397). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets P-selectin glycoprotein ligand 1 (gene Selplg) in mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
P-selectin glycoprotein ligand-1 (PSGL-1, SELPLG) is a mucin-like glycoprotein on leukocytes that binds P-, E- and L-selectin. These calcium-dependent interactions capture leukocytes from the bloodstream and start their rolling along inflamed vessels, and PSGL-1 also acts as a receptor for enterovirus 71.
| Target | P-selectin glycoprotein ligand 1 (gene Selplg; UniProt Q62170, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Mouse, Rat) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Mouse) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant mouse PSGL-1/Selplg fragment (Leu18–Pro397), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places P-selectin glycoprotein ligand 1 in the cell membrane. Tissue expression noted by UniProt (mouse): Highly expressed in blood, bone marrow, brain, adipose tissue, spleen, and thymus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
