| Field | Specification |
|---|---|
| Target | |
| Alternative names | Polypyrimidine tract-binding protein 1; PTB; 57 kDa RNA-binding protein PPTB-1; Heterogeneous nuclear ribonucleoprotein I; hnRNP I; PTBP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PTBP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AFEO-16 is a rabbit monoclonal antibody that recognizes polypyrimidine tract-binding protein 1 (gene PTBP1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 57.2 kDa, and the supplier reports an observed band at about 57 kDa.
PTBP1 (also called hnRNP I) is an RNA-binding protein that binds pyrimidine-rich sequences in introns and represses inclusion of many alternative exons. It shapes splicing programs across tissues and also regulates mRNA stability and IRES-driven translation, and its loss is linked to neuronal and muscle differentiation.
| Target | Polypyrimidine tract-binding protein 1 (gene PTBP1; UniProt P26599, human) |
|---|---|
| Clone | AFEO-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.2 kDa |
| Observed MW | 57 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human PTBP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places polypyrimidine tract-binding protein 1 in the nucleus and cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
