| Field | Specification |
|---|---|
| Target | |
| Alternative names | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN;3.1.3.16;3.1.3.48;3.1.3.67;Mutated in multiple advanced cancers 1;Phosphatase and tensin homolog;PTEN;MMAC1, TEP1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PTEN |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BEI-16 is a rabbit monoclonal antibody that recognizes phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 47.2 kDa, and the supplier reports an observed band at about 54 kDa.
PTEN is a lipid and protein phosphatase that removes the 3-phosphate from PIP3, directly opposing PI3K and dampening AKT signaling. By restraining this pathway it limits cell growth, survival and proliferation, and it is one of the most frequently inactivated tumor suppressors in human cancer.
| Target | Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (gene PTEN; UniProt P60484, human) |
|---|---|
| Clone | BEI-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.2 kDa |
| Observed MW | 54 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human HeLa cells, human 293T cells, human MCF-7 cells, rat liver tissue, mouse heart tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PTEN.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN in the cytoplasm, nucleus, cell projection and postsynaptic density. Tissue expression noted by UniProt (human): Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Xuebiao et al. (2025) Feedback loop centered on MAF1 reduces blood–brain barrier damage in sepsis-associated encephalopathy. Cellular & Molecular Biology Letters. 10.1186/s11658-025-00686-x
Mengge Sun et al. (2019) Circulating MicroRNA-19b Identified From Osteoporotic Vertebral Compression Fracture Patients Increases Bone Formation. Journal of Bone and Mineral Research. 10.1002/jbmr.3892
Lijia Wen et al. (2024) PTEN Deficiency Induced by Extracellular Vesicle miRNAs from Clonorchis sinensis Potentiates Cholangiocarcinoma Development by Inhibiting Ferroptosis. International Journal of Molecular Sciences. 10.3390/ijms251910350
Huiru Liu et al. (2024) Oxidized DJ-1 activates the p-IKK/NF-κB/Beclin1 pathway by binding PTEN to induce autophagy and exacerbate myocardial ischemia-reperfusion injury. European Journal of Pharmacology. 10.1016/j.ejphar.2024.176496
Yan Ping Wei et al. (2018) Matrine exerts inhibitory effects in melanoma through the regulation of miR-19b-3p/PTEN. International Journal of Oncology. 10.3892/ijo.2018.4414
Zhenze Lu et al. (2021) Long non-coding RNA LOC366613 alleviates the cerebral ischemic injury via regulating the miR-532-5p/phosphatase and tensin homolog axis. Bioengineered. 10.1080/21655979.2021.1930966
Chaoyang Zhu et al. (2015) Prognostic role of PPAR-γ and PTEN in the renal cell carcinoma. International Journal of Clinical and Experimental Pathology. PMID: 26722456
Shuchao Wang et al. (2018) Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons. Annals of Anatomy-Anatomischer Anzeiger. 10.1016/j.aanat.2018.08.005
Dong Wang et al. (2019) Participation of tumor suppressors long non-coding RNA MEG3, microRNA-377 and PTEN in glioma cell invasion and migration. Pathology Research and Practice. 10.1016/j.prp.2019.152558
Shu-Wen Sun et al. (2017) Expression and clinical significance of EGR-1 and PTEN in the pituitary tumors of elderly patients. Oncology Letters. 10.3892/ol.2017.6375
