| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Protein tyrosine phosphatase type IVA 2;3.1.3.48;HU-PP-1;OV-1;PTP (CAAXII);Protein-tyrosine phosphatase 4a2;Protein-tyrosine phosphatase of regenerating liver 2;PRL-2;PTP4A2;PRL2, PTPCAAX2;BM-008; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human PTP4A2, identical to the related mouse and rat sequences. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of PTP4A2 (Protein tyrosine phosphatase type IVA 2) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-PTP4A2 Antibody Picoband® catalog # PB9740. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human PTP4A2, identical to the related mouse and rat sequences.
- Molecular weight context: reported MW: 19 kDa; calculated MW: 19127 MW
- Reactivity: Human,Rat
- Applications: Flow Cytometry, IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Protein tyrosine phosphatase type IVA 2; Protein tyrosine phosphatase type IVA 2. Protein tyrosine phosphatase type IVA 2 is an enzyme that in humans is encoded by the PTP4A2 gene. The protein encoded by this gene belongs to a small class of the protein tyrosine phosphatase (PTP) family. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. PTPs in this class contain a protein tyrosine phosphatase catalytic domain and a characteristic C-terminal prenylation motif. This PTP has been shown to primarily associate with plasmic and endosomal membrane through its C-terminal prenylation. This PTP was found to interact with the beta-subunit of Rab geranylgeranyltransferase II (beta GGT II), and thus may function as a regulator of GGT II activity. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which suggested its role in tumorigenesis. Alternatively spliced transcript variants have been described. Related pseudogenes exist on chromosomes 11, 12 and 17. Functional note: Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Promotes tumors. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB. . Reported localization: Cell membrane. Early endosome. Cytoplasm. Expression/tissue context: Ubiquitously expressed, with highest levels in skeletal muscle, heart and thymus. Overexpressed in prostate tumor tissue. .
Research relevance and current trends
- Cancer: Researchers commonly examine how PTP4A2 (Protein tyrosine phosphatase type IVA 2) relates to this theme using model systems and orthogonal readouts.
- Cell Cycle: Researchers commonly examine how PTP4A2 (Protein tyrosine phosphatase type IVA 2) relates to this theme using model systems and orthogonal readouts.
- Kinases/Phosphatases: Researchers commonly examine how PTP4A2 (Protein tyrosine phosphatase type IVA 2) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative PTP4A2 (Protein tyrosine phosphatase type IVA 2) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of PTP4A2 (Protein tyrosine phosphatase type IVA 2) across tissue regions and cell types using matched controls.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
