| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pumilio homolog 1; HsPUM; Pumilio-1; PUM1; KIAA0099; PUMH1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human PUM1 recombinant protein (Position: M1-D361). |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes pumilio homolog 1 (gene PUM1) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
Pumilio homolog 1 (PUM1) is a PUF-family RNA-binding protein that recognizes the UGUANAUA motif in mRNA 3′ UTRs. By recruiting deadenylase complexes and other repressors it lowers the stability and translation of its target mRNAs, with roles in neuronal function, stem cells and fertility.
| Target | Pumilio homolog 1 (gene PUM1; UniProt Q14671, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human PUM1 fragment (Met1–Asp361), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places pumilio homolog 1 in the cytoplasm and cytoplasmic granule. Tissue expression noted by UniProt (human): Expressed in brain, heart, kidney, muscle, intestine and stomach.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
