| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bcl-2-binding component 3;JFY-1;p53 up-regulated modulator of apoptosis;BBC3;PUMA; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PUMA |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone DDO-2 is a rabbit monoclonal antibody that recognizes bcl-2-binding component 3, isoforms 1/2 (gene BBC3) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 20.5 kDa, and the supplier reports an observed band at about 21 kDa.
PUMA (BBC3) is a BH3-only pro-apoptotic Bcl-2 family protein. It is strongly induced by p53 after DNA damage and also by p53-independent stresses, and it binds and neutralizes anti-apoptotic proteins such as Bcl-2 and Bcl-xL, freeing BAX and BAK to trigger mitochondrial apoptosis.
| Target | Bcl-2-binding component 3, isoforms 1/2 (gene BBC3; UniProt Q9BXH1, human) |
|---|---|
| Clone | DDO-2 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.5 kDa |
| Observed MW | 21 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human HepG2 cells, human K562 cells, human HeLa cells, human Caco-2 cells, rat C6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human breast cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human PUMA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places bcl-2-binding component 3, isoforms 1/2 in the mitochondrion. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wei Yang et al. (2023) Ti3C2 (MXene) nanosheets disrupt spermatogenesis in male mice mediated by the ATM/p53 signaling pathway. Biology Direct. 10.1186/s13062-023-00382-w
Yu Zhao et al. (2011) Introduction of hypoxia-targeting p53 fusion protein for the selective therapy of non-small cell lung cancer. Cancer Biology & Therapy. 10.4161/cbt.11.1.13960
Lian Hui et al. (2015) Nerve growth factor reduces apoptotic cell death in rat facial motor neurons after facial nerve injury. Neurosciences. PMID: 25630785
