| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pumilio homolog 1; HsPUM; Pumilio-1; PUM1; KIAA0099; PUMH1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Pumilio 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 19P55 is a rabbit monoclonal antibody that recognizes pumilio homolog 1 (gene PUM1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 126.5 kDa, and the supplier reports an observed band at about 140 kDa.
Pumilio homolog 1 (PUM1) is a PUF-family RNA-binding protein that recognizes the UGUANAUA motif in mRNA 3′ UTRs. By recruiting deadenylase complexes and other repressors it lowers the stability and translation of its target mRNAs, with roles in neuronal function, stem cells and fertility.
| Target | Pumilio homolog 1 (gene PUM1; UniProt Q14671, human) |
|---|---|
| Clone | 19P55 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 126.5 kDa |
| Observed MW | 140 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:60 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human pumilio 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places pumilio homolog 1 in the cytoplasm and cytoplasmic granule. Tissue expression noted by UniProt (human): Expressed in brain, heart, kidney, muscle, intestine and stomach. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
