| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Steroid hormone receptor ERR1;Estrogen receptor-like 1;Estrogen-related receptor alpha;ERR-alpha;Nuclear receptor subfamily 3 group B member 1;ESRRA;ERR1, ESRL1, NR3B1; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Gene ID | |
| Host | |
| Immunogen | A synthesized peptide derived from human Pumilio 2 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-PUM2 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23P75; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-Pumilio 2 Rabbit Monoclonal Antibody catalog # M03347. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: PUM2 (Steroid hormone receptor ERR1).
- Antibody format: Monoclonal; clone 23P75; isotype IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
PUM2 (protein: T-cell surface glycoprotein CD3 zeta chain) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism. Induces the expression of PERM1 in the skeletal muscle. . Reported cellular localization context: Nucleus . Tissue expression notes (as provided): Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Domain Families,Epigenetics and Nuclear Signaling,Metabolic Signaling Pathways,Metabolism,Mitochondrial Metabolism,Nuclear Hormone Receptors,Nuclear Receptors,Nuclear Signaling Pathways,Nucleotide Metabolism,Pathways and Processes,Signal Transduction,Signaling Pathway,Transcription,Zinc Finger.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
- Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.
Workflow ideas (metafield): Validate PUM2 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect PUM2 expression by Western blot in cell or tissue lysates, Localize PUM2 by immunofluorescence/immunocytochemistry in cultured cells, Quantify PUM2-positive cells by flow cytometry in single-cell suspensions, Enrich PUM2 by immunoprecipitation from lysates for downstream analysis
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 114 kDa; calculated MW: 45510 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 114 kDa
- Cellular localization (provided): Nucleus .
- Tissue details (provided): Visceral organs specific expression. Strong expression was found in liver, kidney and intestine followed by spleen and to a lesser extent the adrenals.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
