| Field | Specification |
|---|---|
| Target | |
| Alternative names | Periodic tryptophan protein 1 homolog; Keratinocyte protein IEF SSP 9502; PWP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human PWP1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30P43 is a rabbit monoclonal antibody that recognizes periodic tryptophan protein 1 homolog (gene PWP1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 55.8 kDa, and the supplier reports an observed band at about 75 kDa.
PWP1 is a WD-repeat chromatin-associated protein involved in ribosome biogenesis. It supports RNA polymerase I transcription of rRNA genes and helps maintain the epigenetic state of rDNA, linking it to cell growth.
| Target | Periodic tryptophan protein 1 homolog (gene PWP1; UniProt Q13610, human) |
|---|---|
| Clone | 30P43 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 55.8 kDa |
| Observed MW | 75 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human PWP1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places periodic tryptophan protein 1 homolog in the nucleus and chromosome. Tissue expression noted by UniProt (human): High levels seen in the placenta, skeletal muscle, kidney and pancreas while lower levels were seen in the heart, brain and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
