| Field | Specification |
|---|---|
| Target | |
| Alternative names | Pygopus homolog 2;PYGO2;PP7910; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Pygopus 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABI-16 is a rabbit monoclonal antibody that recognizes pygopus homolog 2 (gene PYGO2) in human and mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 41.2 kDa, and the supplier reports an observed band at about 50 kDa.
Pygopus homolog 2 (PYGO2) is a nuclear PHD-finger protein that works with BCL9 and β-catenin to support Wnt target gene transcription. It also binds methylated histone H3 lysine 4, and it is studied in development, stem cell maintenance and cancer.
| Target | Pygopus homolog 2 (gene PYGO2; UniProt Q9BRQ0, human) |
|---|---|
| Clone | ABI-16 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.2 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human MCF-7 cells, human SH-SY5Y cells, human PC-3 cells, huamn U2OS cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human uterus tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human pygopus 2.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places pygopus homolog 2 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
