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| Alternative Names | Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27 |
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Overview
Anti-QRFPR/GPR103 (extracellular) Antibody is an antibody targeting Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27 Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27 (also reported as Pyroglutamylated RF-amide peptide receptor, Orexigenic neuropeptide QRFP receptor, G-protein coupled receptor 103, Peptide P518 receptor, SP9155, AQ27).
- Immunogen/epitope region: Extracellular, N-terminus.
- Homology note: Mouse - identical; human - 13/17 amino acid residues identical (informative for cross-species interpretation).
- Species reactivity (as provided): Human, Rat, Mouse.
- Lot quality control (as provided): Western blot analysis.
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
QRFP is a 26-amino acid peptide (QRFP-26) that, widely distributed among vertebrates, is a member of the RF amide peptide family harboring the arginine-phenylalanine-amide (Arg-Phe-NH2) sequence motif in their carboxyl terminal. It is the cognate ligand of the human orphan receptor GPR103, a G protein-coupled receptor. Like other members of the super family, QRFP receptor has an extracellular N-terminal tail and intracellular C-terminus.Its QRFP N-terminus binds with high affinity to GPR103 and has the efficacy to inhibit cAMP synthesis.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-GR033.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-GR033; Negative control: BLP-GR033.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
