| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAB27B; Ras-related protein Rab-27B; EC 3.6.5.2; C25KG |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RAB27B, which shares 95.5% amino acid (aa) sequence identity with both mouse and rat RAB27B. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ras-related protein Rab-27B (gene RAB27B) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry.
Rab27B (RAB27B) is a Rab GTPase closely related to Rab27A that regulates regulated secretion and late endosome trafficking. It controls release of granules and exosomes in several cell types and supports axonal transport of the BDNF receptor TrkB.
| Target | Ras-related protein Rab-27B (gene RAB27B; UniProt O00194, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human RAB27B. Sequence identity with the mouse and rat orthologs: 95.5%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ras-related protein Rab-27B in the membrane and late endosome. Tissue expression noted by UniProt (human): Expressed primarily in testis.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xue Guan et al. (2025) pH-Responsive 2D Niobium Carbide Nanosheets for Targeted circPUM1 siRNA Delivery in Ovarian Cancer Therapy. Materials Today Bio. 10.1016/j.mtbio.2025.102314
