Anti-Rab4 RAB4A Rabbit Monoclonal Antibody

SKU:BHA21007799
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-RAB4A antibody (Rabbit monoclonal, clone CCI-18, Rabbit IgG). Validated for Western blot, immunofluorescence and flow cytometry. Reactivity: Human, Mouse, Rat. Recognizes ras-related protein Rab-4A. Observed band in Western blot: about 24 kDa.
Target RAB4A
Clone number CCI-18
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, ICC, IF, Flow Cytometry
Options selector
Catalog no. Size Conjugation
M04643-1 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB,ICC,IF,Flow Cytometry
      Application details: WB 1:500-1:2000
      ICC/IF 1:50-1:200
      FC 1:50
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target RAB4A
Alternative names Ras-related protein Rab-4A;RAB4A;RAB4;
UniProt # P20338
Host Rabbit
Clone CCI-18
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunofluorescence
  • Flow Cytometry
Immunogen A synthesized peptide derived from human Rab4
Molecular weight 24.4 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Membrane; Peripheral membrane protein. Cytoplasm. Generally associated with membranes. Cytoplasmic when phosphorylated by CDK1.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M04643-1
Main SKU BHA21007799
Rabbit Monoclonal

Product Overview

Clone CCI-18 is a rabbit monoclonal antibody that recognizes ras-related protein Rab-4A (gene RAB4A) in human, mouse and rat samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 24.4 kDa, and the supplier reports an observed band at about 24 kDa.

Rab4A (RAB4A) is a Rab GTPase on early endosomes that controls a fast route for returning internalized receptors to the cell surface. It influences VEGFR2 endosomal trafficking and signaling and platelet granule release.

Target Ras-related protein Rab-4A (gene RAB4A; UniProt P20338, human)
Clone CCI-18
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 24.4 kDa
Observed MW 24 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunocytochemistry / Immunofluorescence 1:50–1:200
Flow cytometry 1:50

Samples with confirmed staining (ICC/IF): HeLa cells.

Immunogen

Synthetic peptide based on the sequence of human rab4.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places ras-related protein Rab-4A in the membrane, cytoplasm, early endosome membrane and recycling endosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 24.4 kDa; observed band at about 24 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human rab4.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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