| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras-related protein Rab-4A;RAB4A;RAB4; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Rab4A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACF-18 is a rabbit monoclonal antibody that recognizes ras-related protein Rab-4A (gene RAB4A) in human, mouse and rat samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 24.4 kDa, and the supplier reports an observed band at about 24 kDa.
Rab4A (RAB4A) is a Rab GTPase on early endosomes that controls a fast route for returning internalized receptors to the cell surface. It influences VEGFR2 endosomal trafficking and signaling and platelet granule release.
| Target | Ras-related protein Rab-4A (gene RAB4A; UniProt P20338, human) |
|---|---|
| Clone | ACF-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 24.4 kDa |
| Observed MW | 24 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): SH-SY5Y cells, MCF-7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human rab4A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ras-related protein Rab-4A in the membrane, cytoplasm, early endosome membrane and recycling endosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
