| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Ras-related protein Rab-6A; Rab-6; RAB6A; RAB6 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RAB6A, which shares 97.2% amino acid (aa) sequence identity with both mouse and rat RAB6A. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of RAB6A (A disintegrin and metalloproteinase with thrombospondin motifs 4) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-RAB6A Antibody Picoband® catalog # A02911-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the C-terminus of human RAB6A, which shares 97.2% amino acid (aa) sequence identity with both mouse and rat RAB6A.
- Molecular weight context: reported MW: 24 kDa; calculated MW: 136311 MW
- Reactivity: Human,Mouse,Rat
- Applications: IHC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
A disintegrin and metalloproteinase with thrombospondin motifs 4; RAB6A, member RAS oncogene family. Ras-related protein Rab-6A is a protein that in humans is encoded by the RAB6A gene located in the eleventh chromosome. This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) ions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. Functional note: Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Involved in COPI-independent retrograde transport from the Golgi to the ER (PubMed:25962623). Reported localization: Golgi apparatus membrane. Expression/tissue context: Ubiquitous.
Research relevance and current trends
- G Protein Signaling: Researchers commonly examine how RAB6A (A disintegrin and metalloproteinase with thrombospondin motifs 4) relates to this theme using model systems and orthogonal readouts.
- Protein Trafficking: Researchers commonly examine how RAB6A (A disintegrin and metalloproteinase with thrombospondin motifs 4) relates to this theme using model systems and orthogonal readouts.
- Ras Family: Researchers commonly examine how RAB6A (A disintegrin and metalloproteinase with thrombospondin motifs 4) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative RAB6A (A disintegrin and metalloproteinase with thrombospondin motifs 4) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of RAB6A (A disintegrin and metalloproteinase with thrombospondin motifs 4) across tissue regions and cell types using matched controls.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
