| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ras-related protein Rab-7a; RAB7A; RAB7 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human RAB7/RAB7A recombinant protein (Position: K21-E177). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ras-related protein Rab-7a (gene RAB7A) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry, immunoprecipitation and ELISA. The predicted molecular weight is 23.5 kDa, and the supplier reports an observed band at about 23 kDa.
Rab7 (RAB7A) is the main Rab GTPase of late endosomes and lysosomes. Through effectors such as RILP and the HOPS complex it drives endosome maturation, microtubule-based positioning and fusion with lysosomes, and it is also needed for autophagosome–lysosome fusion, making it a standard late endosome marker.
| Target | Ras-related protein Rab-7a (gene RAB7A; UniProt P51149, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 23.5 kDa |
| Observed MW | 23 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse, Rat) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A375 cells, human U-87MG cells, human HEL whole cellrat L6 whole cellmouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human diffuse large B cells lymphoma tissue, human duodenal papilla adenocarcinoma tissue, human endometrioid adenocarcinoma tissue, human glioblastoma tissue, human larynx squamous cells carcinoma tissue, human lung adenocarcinoma tissue, human placenta tissue, human prostate adenocarcinoma tissue, human spleen tissue, human testicular seminoma tissue, mouse kidney tissue, mouse skeletal muscle tissue, rat brain tissue, rat colon tissue, rat kidney tissue, rat lung tissue, rat skeletal muscle tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed result (IP): A431 cells.
Samples with a confirmed signal (flow cytometry): Jurkat cells, HEPA1–6 cells, RH35 cells.
Immunogen
Recombinant human RAB7/RAB7A fragment (Lys21–Glu177), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ras-related protein Rab-7a in the cytoplasmic vesicle, late endosome membrane and lysosome membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
