| Field | Specification |
|---|---|
| Target | |
| Alternative names | RAF proto-oncogene serine/threonine-protein kinase;2.7.11.1;Proto-oncogene c-RAF;cRaf;Raf-1;RAF1;RAF; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Raf1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone BEC-18 is a rabbit monoclonal antibody that recognizes RAF proto-oncogene serine/threonine-protein kinase (gene RAF1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 73.1 kDa, and the supplier reports an observed band at about 73 kDa.
RAF1 (c-Raf) is a serine/threonine kinase that passes signals from active Ras to MEK1/2 and then ERK1/2, a cascade that shapes proliferation, differentiation and survival. Its activity is controlled by phosphorylation at several regulatory residues, and it is studied widely in growth-factor signaling and in Ras-driven cancers.
| Target | RAF proto-oncogene serine/threonine-protein kinase (gene RAF1; UniProt P04049, human) |
|---|---|
| Clone | BEC-18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 73.1 kDa |
| Observed MW | 73 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human K562 cells, human THP-1 cells, human MCF-7 cells, human U251 cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (ICC/IF): human HEK293T cells.
Immunogen
Synthetic peptide based on the sequence of human raf1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places RAF proto-oncogene serine/threonine-protein kinase in the cytoplasm, cell membrane, mitochondrion and nucleus. Tissue expression noted by UniProt (human): In skeletal muscle, isoform 1 is more abundant than isoform 2. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Sining Feng et al. (2021) Effects of combination treatment with transcranial magnetic stimulation and bone marrow mesenchymal stem cell transplantation or Raf inhibition on spinal cord injury in rats. Molecular Medicine Reports. 10.3892/mmr.2021.11934
Huanyue Cui et al. (2021) Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways. Evidence-based Complementary and Alternative Medicine. 10.1155/2021/6103158
Gui-Qiu Zhao et al. (2016) The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epithelial cells against Aspergillus fumigatus infection. International Journal of Ophthalmology. 10.18240/ijo.2016.10.01
