| Field | Specification |
|---|---|
| Target | |
| Alternative names | V; D; J recombination-activating protein 1; RAG-1; RING finger protein 74; RAG1; RNF74 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RAG1, identical to the related mouse and rat sequences. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets V(D)J recombination-activating protein 1 (gene RAG1) in human and mouse samples and is validated for Western blot. The predicted molecular weight is 119.1 kDa, and the supplier reports an observed band at about 119 kDa.
RAG1 is the catalytic subunit of the RAG recombinase that, together with RAG2, cuts DNA at recombination signal sequences to start V(D)J recombination. This process assembles the diverse antibody and T-cell receptor genes of developing lymphocytes, and RAG1 is conserved across jawed vertebrates, including sharks such as the nurse shark.
| Target | V(D)J recombination-activating protein 1 (gene RAG1; UniProt P15918, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 119.1 kDa |
| Observed MW | 119 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse) |
|---|
Samples with a confirmed band (WB): human Jurkat cells, human K562 cells, human A431 cells, mouse thymus tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human RAG1. The peptide sequence is conserved in mouse and rat.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places V(D)J recombination-activating protein 1 in the nucleus. Tissue expression noted by UniProt (human): Maturing lymphoid cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Liu Yongxiang et al. (2026) From PGT-M discovery to mechanism: functional validation of novel compound heterozygous RAG1 mutations in severe combined immunodeficiency. Journal of Assisted Reproduction and Genetics. 10.1007/s10815-026-03887-1
